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Invitrogen
ACSL3 Polyclonal Antibody
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FIGURE: 1 / 5
ACSL3 Antibody (PA5-29507) in ICC/IF
ACSL3 Polyclonal Antibody detects ACSL3 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACSL3 stained by ACSL3 Polyclonal Antibody (Product # PA5-29507) diluted at 1:500.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-29507
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ACSL3. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.81 mg/mL
Amount
81 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat, Raji.
Predicted reactivity: Mouse (95%), Rat (95%), Pig (95%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
An initial reaction in fatty acid metabolism in eukaryotic cells is activation of fatty acids catalyzed by acyl-CoA synthetase. FACL3 (fatty acid CoA ligase, long-chain 3) is identified as member of the acyl-CoA synthetase (ACS) family by PCR of rat brain cDNAs using primers based on the conserved region of the ACS protein. The 720-amino acid rat protein preferentially utilizes myristate, laurate, arachidonate, and eicosapentaenoate, and is expressed primarily in brain. The predicted 720-amino acid FACL3 human protein is 92% identical to that of rat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Arachidonate--CoA ligase; Arachidonate--CoA ligase Acsl3; Brain acyl-CoA synthetase II; Fatty acid CoA ligase Acsl3; fatty acid Coenzyme A ligase, long chain 3; LACS 3; Long-chain acyl-CoA synthetase 3; Long-chain-fatty-acid--CoA ligase 3; Medium-chain acyl-CoA ligase Acsl3
Gene Aliases: 2610510B12Rik; ACS3; ACSL3; C85929; FACL3; LACS3; Pro2194
UniProt ID: (Rat) Q63151, (Mouse) Q9CZW4
Entrez Gene ID: (Rat) 114024, (Mouse) 74205
long-chain fatty acid metabolic process
lipid metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
brain development
response to nutrient
metabolic process
response to organic cyclic compound
very-low-density lipoprotein particle assembly
positive regulation of Golgi to plasma membrane protein transport
long-chain fatty acid import
positive regulation of secretion
positive regulation of phosphatidylcholine biosynthetic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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