Invitrogen

ACTA1 Polyclonal Antibody

Name: ACTA1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-78716

高级验证

2个已发表图片

3 篇参考文献

查看其他25个ACTA1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

ACTA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ACTA1 Antibody (PA5-78716) in IHC (P)

Immunohistochemistry (Paraffin) analysis of Actin in paraffin-embedded section of human colorectal adenocarcinoma tissue using Actin Polyclonal Antibody (Product # PA5-78716). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL diluti... View More

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ACTA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of Actin was observed in Rat Heart, Rat Skeletal Muscle, Mouse Heart and Mouse Skeletal Muscle in comparison to Mouse Kidney and Rat Kidney using Actin Polyclonal Antibody (Product # PA5-78716) in Western Blot. {RE}

Immunogen image

Immunogen Image ACTA1 Polyclonal Antibody

产品信息

PA5-78716

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (359-377aa ITKQEYDEAGPSIVHRKCF).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2745832

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human placenta tissue, human Hela whole cell, human HepG2 whole cell, human 293T whole cell, human A431 whole cell, human U87 whole cell, human U937 whole cell, human K562 whole cell, rat heart tissue, rat brain tissue, rat kidney tissue, mouse heart tissue, mouse brain tissue, mouse spleen tissue, mouse kidney tissue. IHC: human breast cancer tissue, human colorectal adenocarcinoma tissue, human placenta tissue, human tonsil tissue, mouse heart tissue, rat heart tissue.

靶标信息

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

仅用于科研。不用于诊断过程。未经明确授权不得转售。