Invitrogen

ADT2 Polyclonal Antibody

问题与答案

问题与答案

问题与答案

抗体检测数据 (2)

ADT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ADT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ADT2 Antibody (PA5-114310) in IHC (P)

Immunohistochemical analysis of ADT2 in paraffin-embedded rice tissue using a polyclonal antibody (Product #PA5-114310). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room te... View More

ADT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ADT2 Antibody in Western Blot (WB)

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ADT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ADT2 in paraffin-embedded rice tissue using a polyclonal antibody (Product #PA5-114310). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ADT2 Antibody in Western Blot (WB) — Western blot analysis of ADT2 using a ADT2 polyclonal antibody (Product #PA5-114310). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:100 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

产品信息

PA5-114310

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

-

免疫组化（石蜡） (IHC (P))

Assay-dependent

-

产品规格

种属反应

Plant

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide within rice Arogenate dehydratase aa 28-77

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 0.2% BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

This antibody specifically recognizes Arogenate dehydratase in Oryza sativa subspecies japonica (Rice).

靶标信息

Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Arogenate dehydratase/prephenate dehydratase 2, chloroplastic; AtADT2

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基因别名: ADT2; Arabidopsis thaliana arogenate dehydratase 2; arogenate dehydratase 2; AT3G07630; AtADT2; MLP3.8; PDT2

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UniProt ID: (Arabidopsis) Q9SSE7

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Entrez Gene ID: (Arabidopsis) 819954

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prephenate dehydratase activity arogenate dehydratase activity dehydratase

L-phenylalanine biosynthetic process