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Invitrogen
AFF4 Monoclonal Antibody (8G12)
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AFF4 Antibody (MA5-49267) in WB
Western blot analysis of AFF4 using AFF4 antibody (Product # MA5-49267). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. Lane 1: human HELA whole cell lysates; Lane 2: human HEPG2 whole cell lysates; Lane 3: human CACO-2 whole cell lysates; Lane 4: human HEK293 whole cell lysates; Lane 5: human MDA-MB-453 whole cell lysates; Lane 6: human PANC-1 whole cell lysates; Lane 7: human SW620 whol... View More
产品信息
MA5-49267
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
8G12
抗原
A synthetic peptide corresponding to a sequence at the N-terminus of human AFF4 (6-53aa RNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQ).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Immunogen sequence is identical to the related mouse sequence.
Positive Control - WB: human HELA whole cell, human HEPG2 whole cell, human CACO-2 whole cell, human HEK293 whole cell, human MDA-MB-453 whole cell, human PANC-1 whole cell, human SW620 whole cell. Flow: 293T cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AF4/FMR2 family member 4; AF4/FMR2 family, member 4; ALL1-fused gene from chromosome 5q31 protein; Major CDK9 elongation factor-associated protein; Protein AF-5q31
基因别名: AF5Q31; AFF4; CHOPS; HSPC092; MCEF
UniProt ID: (Human) Q9UHB7
Entrez Gene ID: (Human) 27125
