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Agrisera
AGO1-PAZ/Argonaute 1 PAZ domain Polyclonal Antibody
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AGO1-PAZ/Argonaute 1 PAZ domain Antibody (AS14-2797) in WB
Total proteins of Chlamydomonas reinhardtii protein saturated in 8 M urea were separated on 15% SDS-PAGE and blotted for 1 hour to 0.2 µm nitrocellulose at 100 V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1 hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H 3) at a dilution of 1:2,500 for an hour at RT with agitation. The blots were washed w... View More
产品信息
AS14-2797
产品规格
种属反应
Algae
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from AGO1-PAZ domain of Chlamydomonas reinhardtii.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Chlamydomonas reinhardtii
靶标信息
Argonaute 1-PAZ encodes the PAZ (Piwi/Argonaute/Zwille) RNA-binding module found within Argonaute-family effector proteins, which localize predominantly to the cytoplasm as core components of the RNA-induced silencing complex (RISC) and can also participate in nuclear RNA-directed silencing. Structurally, the PAZ region is a compact globular fold built around a left-handed six-stranded beta-barrel capped by two alpha-helices and augmented by a distinctive beta-hairpin/short alpha-helix appendage that helps form an RNA-binding pocket. Functionally, the PAZ domain binds the 3-prime end of small RNAs (miRNAs/siRNAs), helping position guide RNAs within Argonaute and supporting key steps in RISC maturation and activity; genetic and biochemical studies indicate that PAZ integrity is particularly important for slicing-independent RISC activation, where it promotes passenger-strand ejection/unwinding and formation of a functional, single-stranded guide-containing complex.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99
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