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Agrisera
AGO10/Argonaute 10 Polyclonal Antibody
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AGO10/Argonaute 10 Antibody (AS15-3071) in WB
50 µg of total protein from Arabidopsis thaliana inflorescences were extracted with extraction buffer (50 m M Tris p H 7.5; 150 m M Na Cl; 1 m M EDTA; 10% v/v Glycerin; 1 m M DTT, 1 x Complete Protease Inhibitor Cocktail, Roche) and denatured with LaemmLi buffer at 95ºC 5 min. were separated on 10% SDS-PAGE and blotted 1.5 h to PVDF using tank transfer. Blots were blocked with blocking buffer (3% milk powder; 1 x TBS; 0.1% Tween-20) 1 h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:10,000 ON at 4ºC wit... View More
产品信息
AS15-3071
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO10 protein sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
Argonaute 10 (AGO10) encodes a cytoplasmic/nucleocytoplasmic Argonaute-family RNA-binding protein that functions within small RNA-directed silencing pathways and is particularly important in meristematic and organ polarity contexts. AGO10 is a multidomain, bilobed protein built around the conserved Argonaute core architecture comprising an N-terminal region followed by structured linker segments that position the PAZ, MID, and PIWI domains; the PAZ domain forms a pocket that recognizes the 3' end of small RNAs, the MID domain helps anchor the 5' end, and the PIWI domain adopts an RNase H-like fold associated with endonucleolytic "slicer" potential in Argonautes. Biologically, Arabidopsis AGO10 is a key regulator of shoot apical meristem maintenance and leaf adaxial-abaxial patterning by preferentially binding and sequestering miR165/166, thereby limiting their availability to AGO1-containing effector complexes and relieving repression of class III HD-ZIP transcripts; genetic and biochemical data support a primary "decoy/sequestration" role for AGO10's small-RNA binding activity in development, with catalytic activity not being the essential requirement for this function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ARGONAUTE 10; ARGONAUTE PROTEIN 10; AT5G43810; MQD19.17; MQD19_17; PINHEAD; PNH; ZLL; ZWILLE
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