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Agrisera
AHA2 p/ATPase 2 phosphorylated, plasma membrane-type Polyclonal Antibody
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AHA2 p/ATPase 2 phosphorylated, plasma membrane-type Antibody (AS22-4789) in WB
Samples: 1 - Recombinant protein MBP-C terminal region of AHA 2, phosphorylation treatment 2 - Recombinant protein MBP-C terminal region of AHA 2, subsequently dephosphorylated 3 - Recombinant protein MBP, phosphorylation treatment 4 - Recombinant protein MBP-C terminal region of AHA 2, Thr 947 Ala, phosphorylation treatment 5- Mark: MW markers 1 µg/well of recombinant protein produced in E. coli and purifi... View More
产品信息
AS22-4789
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana AHA2 protein sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Amaranthus cruentus, Arabidopsis thaliana, Beta vulgaris
靶标信息
AHA2 encodes a P-type plasma membrane H+-ATPase that localizes to the plant cell plasma membrane, where it functions as a primary electrogenic proton pump exporting H+ from the cytosol to the apoplast to build the proton-motive force that energizes secondary transport, supports nutrient uptake, and contributes to apoplastic acidification linked to cell expansion and root growth. The AHA2 protein is an integral membrane ATPase with multiple transmembrane helices flanking a large cytosolic catalytic core that contains the conserved P-type ATPase phosphorylation machinery, and it carries regulatory cytosolic terminal regions; its C-terminal tail forms an autoinhibitory regulatory domain whose inhibition can be relieved by phosphorylation of the penultimate threonine and subsequent binding of 14-3-3 proteins at the extreme C-terminal motif, thereby increasing pump activity. Genetic and physiological analyses support a major role for AHA2-driven H+ efflux in roots, including regulation of extracellular pH homeostasis and root elongation (notably in the elongation zone), with AHA2 loss-of-function reducing root proton secretion and altering root developmental responses under both standard and nutrient-stress conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AHA2; AT4G30190; AtHA2; F9N11.40; F9N11_40; H(+)-ATPase 2; HA2; P-TYPE H(+)-ATPASE ISOFORM 2; PLASMA MEMBRANE PROTON ATPASE 2; PMA2
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