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Agrisera
AHK2/Histidine kinase 2 Polyclonal Antibody
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AHK2/Histidine kinase 2 Antibody (AS12-2113) in WB
The Arabidopsis thaliana wild-type (WS) and ahk 2-2 T-DNA insertion mutant plants were grown on MS/2 media for six days. Tissue was collected, frozen in liquid nitrogen, ground in 3 volumes of 2 x SDS-PAGE loading buffer and heated at 95 C for 5 min. After 10 min centrifugation at RT, 20 µL of the supernatant was loaded per lane on a 7.5% TGX gel (Bio-Rad). After separation, proteins were blotted 1 hr onto ... View More
产品信息
AS12-2113
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AHK2 protein sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
AHK2 encodes a membrane-bound hybrid sensor histidine kinase cytokinin receptor in Arabidopsis thaliana that is found predominantly on endomembranes, with strongest evidence for localization to the endoplasmic reticulum. The protein is a single-pass/multi-pass transmembrane receptor-like kinase with an N-terminal extracytosolic sensory region containing a ligand-binding CHASE domain, followed by transmembrane segments that anchor it in the membrane and a C-terminal cytosolic signaling module comprising the conserved histidine kinase core (including the phosphoacceptor and ATPase subdomains) fused to a receiver (REC) domain, enabling two-component style phosphotransfer. Functionally, AHK2 binds bioactive cytokinins (with high affinity reported for isopentenyladenine and trans-zeatin) to promote receptor autophosphorylation and initiation of a multistep His-to-Asp phosphorelay through histidine phosphotransfer proteins to response regulators, thereby regulating cytokinin-dependent transcriptional programs that influence meristematic activity, organ growth, root and shoot development, and physiological stress responses, acting partially redundantly but with distinct spatial activity compared with the other cytokinin receptors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AHK2; AT5G35750; histidine kinase 2; HK2; MXH1.16; MXH1_16
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