Invitrogen

AHNAK Recombinant Rabbit Monoclonal Antibody (PSH01-10)

Name: AHNAK Recombinant Rabbit Monoclonal Antibody (PSH01-10)
Brand: Invitrogen
SKU: MA5-49610

高级验证

查看其他11个AHNAK抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (14)
高级验证 (3)

AHNAK Antibody in Immunocytochemistry (ICC/IF)

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AHNAK Antibody (MA5-49610) in ICC/IF

Immunofluorescence analysis of AHNAK was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with AHNAK Rabbit Recombinant Monoclonal Antibody (PSH01-10) (Product # MA5-49610) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa F... View More

AHNAK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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AHNAK Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of AHNAK was observed in high expressing cell lines such as U-87 MG, PANC-1, 769-P, NIH:OVCAR-3, HaCaT, SW480 in comparison with low expressing cell lines such as A-673 and Jurkat using AHNAK Rabbit Recombinant Monoclonal Antibody (PSH01-10) (Product # MA5-49610) in Western Blot. {RE}

Immunogen image

Immunogen Image AHNAK Recombinant Rabbit Monoclonal Antibody (PSH01-10)

产品信息

MA5-49610

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:5,000-1:20,000

免疫细胞化学 (ICC/IF)

1:100-1:250

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH01-10

抗原

Recombinant protein within human AHNAK aa 451-900/5,890.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_3092422

产品详细信息

Sequence Similarities: 100% Mouse/Rat.

Tissue Specificity: Low tissue specificity.

Positive Control: Human lung carcinoma tissue, human brain tissue, mouse brain tissue, rat brain tissue, A431, HeLa, NIH/3T3.

Subcellular Location: Nucleus.

Predicted band size: 629 kDa.

靶标信息

AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。