Invitrogen

AIM2 Polyclonal Antibody, eBioscience™

Name: AIM2 Polyclonal Antibody, eBioscience\u2122
Brand: Invitrogen
SKU: 14-6008-93

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12 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

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AIM2 Antibody (14-6008-93) in WB

Cell lysates prepared from Jurkat cells (lane 1) and mouse macrophages left untreated (lane 2) or treated for 12 hr with IFN gamma and LPS (lane 3) were immunoblotted with a 1:1000 dilution of the Anti-AIM2 polyclonal antibody. Bands were visualized using Anti-Rabbit IgG HRP.

AIM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AIM2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

AIM2 Antibody — Inflammasome activation in AIM2 knockdown THP-1 cells after EBV infection. The following analyses were performed at 24 hours after incubation with RPMI (mock) or AGS-EBV-GFP cell supernatant (AGSGFP). (A) AIM2 mRNA expression of siRNA-treated THP-1 cells. The asterisk (*) indicates p = 0.02 (B) Immunoblot analysis of siRNA-treated THP-1 cells. Β-actin was blotted as a loading control. (C) mRNA expression of caspase-1 and IL-1Β in siRNA-treated THP-1 cells. The double asterisk (**) indicates p<0.001 by one-way ANOVA with Tukey’s post hoc test. (D) IL-1Β concentration in siRNA-treated THP-1 cells. (A-D) The data are expressed as fold changes compared to si-control-treated THP-1 cells incubated with RPMI (mock). (A, C, D) The data represent one experiment with triplicate samples. The error bars represent S.E. {KD}

产品信息

14-6008-93

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10670279

产品详细信息

Description: This polyclonal antibody reacts with human, mouse, and rat AIM2 (also known as Antigen isolated from Immunoselected Melanoma 2). A member of the HIN-2000 family, this 40-kDa cytoplasmic protein is expressed in the spleen, small intestine, peripheral blood lymphocytes, and testis. Moreover, AIM2 is expressed in tumors of the neuroectodermis, breast, ovary, and colon. As a component of the inflammosome, AIM2 detects double-stranded viral and bacterial DNA present in the cytosol and initiates Caspase1 activation. Expression of AIM2 can be induced by interferons.

Applications Reported: This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested: This polyclonal antibody has been tested by immunoblotting of Jurkat cells and mouse macrophages treated for 12 hrs with IFN gamma and LPS. This can be used at less than or equal to a 1:1000 dilution. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Absent in melanoma 2; Ifi-210; Interferon-inducible protein 210; Interferon-inducible protein AIM2; Interferon-inducible protein p210; RP11-520H16.3

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基因别名: AIM2; Gm1313; Ifi210; PYHIN4

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UniProt ID: (Human) O14862, (Mouse) Q91VJ1

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Entrez Gene ID: (Human) 9447, (Rat) 304987, (Mouse) 383619

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功能

double-stranded DNA binding protein binding identical protein binding molecular_function DNA binding DNA-binding transcription factor

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