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Agrisera
AKINB1/SNF1-related protein kinase regulatory subunit beta-1 Polyclonal Antibody
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AKINB1/SNF1-related protein kinase regulatory subunit beta-1 Antibody (AS09-460) in WB
25 µg of empty vector (1), p CDNA 3.1 – HA- tagged AKIN-beta 1 (2), p CDNA 3.1 – HA- tagged AKIN-beta 2 (3), 50 µg of total protein from Arabidopsis thaliana leaves had been homogenized into 50 m M Na PO 4 p H 7.5,20 m M KCl, 0.5 M sucrose, 0.2 m M PMSF, 10 m M DTT and protease inhibitor cocktail (Sigma, P 9599). Extracts were separated on 10%Nu Page Bis-Tris Novex SDS PAGE (Invitrogen) gels followed by a t... View More
产品信息
AS09-460
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-1.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
200 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
AKINB1 (AKINbeta1) encodes a non-catalytic regulatory beta subunit of the Arabidopsis SnRK1 energy-sensing protein kinase complex and is predominantly a soluble intracellular protein that partitions among the cytoplasm and nucleus but is also targeted to the Golgi apparatus in a manner influenced by N-myristoylation. Functionally, AKINB1 serves as a scaffold/adaptor within the heterotrimeric SnRK1 complex, promoting assembly and tuning subcellular localization of kinase complexes with the catalytic alpha and regulatory gamma-type subunits, thereby shaping downstream signaling outputs under changing nutrient and environmental conditions. Consistent with conserved beta-subunit architecture described for SnRK1/AMPK/SNF1 families, AKINB1 is characterized by interaction modules that mediate binding to other SnRK1 subunits and contribute to substrate targeting, and genetic perturbation of AKINB1 alters central metabolism and transcriptional programs, including effects on organic acid (TCA cycle-associated) metabolism and broad modulation of genes involved in carbon, lipid, and nitrogen metabolism and sugar-responsive regulation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AKINB1; AKINbeta1; AT5G21170; KINB1; KINbeta1; T10F18.200; T10F18_200
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