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Invitrogen
AKR1D1 Polyclonal Antibody
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AKR1D1 Antibody (PA5-95612) in WB
Western blot analysis of AKR1D1 in Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse testis tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with AKR1D1 polyclonal antibody (Product # PA5-95612) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HR... View More
产品信息
PA5-95612
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence of human AKR1D1 (EEMKDIEALNKNVRFVELLMWRDHPEYPFHDEY).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human CACO-2 whole cell, rat liver tissue, mouse liver tissue. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 3-oxo-5-beta-steroid 4-dehydrogenase; Aldo-keto reductase family 1 member D1; aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase); delta 4-3-ketosteroid-5-beta-reductase; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase; steroid-5-beta-reductase, beta polypeptide 1 (3-oxo-5 beta-steroid delta 4-dehydrogenase beta 1)
基因别名: 3o5bred; AKR1D1; CBAS2; SRD5B1
UniProt ID: (Human) P51857, (Mouse) Q8VCX1, (Rat) P31210
Entrez Gene ID: (Human) 6718, (Mouse) 208665, (Rat) 192242
