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Invitrogen
ALG1 Polyclonal Antibody
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ALG1 Antibody (PA1-031) in WB
Western blot analysis of Alg-1 was performed by loading 15 µg WT or Alg-1 (-) worm extract onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing Alg-1 (Product # PA1-031) at a dilution of 1:5000 for 1 hour and room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution o... View More
产品信息
PA1-031
应用
建议稀释比
已发表文章
产品规格
种属反应
C. elegans
已发表种属
C. elegans
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide sequence DLEEIPNSPPTQPQTFSDVPQR
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA, 30% glycerol
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
nematode larval development
embryo development ending in birth or egg hatching
gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
mRNA cleavage involved in gene silencing by miRNA
vulval development
regulation of development, heterochronic
hermaphrodite genitalia development
regulation of cell fate specification
small RNA loading onto RISC
RNA phosphodiester bond hydrolysis, endonucleolytic
