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Invitrogen
AMACR Polyclonal Antibody
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AMACR Antibody (PA5-95365) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of AMACR in U2OS cells using AMACR Polyclonal Antibody (Product # PA5-95365). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter set... View More
产品信息
PA5-95365
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence in the middle region of human AMACR (208-246aa RGQNMLDGGAPFYTTYRTADGEFMAVGAIEPQFYELLIK).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell lysates, human LNCAP whole cell lysates, human HepG2 whole cell lysates, human Hela whole cell lysates, rat kidney tissue lysates, rat liver tissue lysates, mouse kidney tissue lysates, mouse liver tissue lysates. IHC: human stomach cancer tissue, mouse kidney tissue, rat kidney tissue. ICC/IF: U2OS cell. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
P504S/AMACR/a-Methylacyl-CoA Racemase is an essential enzyme in the b-oxidation of branched-chain fatty acids. High expression of P504S protein is found in prostatic adenocarcinoma but not in benign prostate tissue by immunohistochemical staining in paraffin-embedded tissue. The expression of P504S is also detected in two premalignant lesions of the prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 2-arylpropionyl-CoA epimerase; 2-methylacyl-CoA racemase; Alpha-methylacyl-CoA racemase; alpha-methylacyl-Coenzyme A racemase; amcr; OTTHUMP00000219978; OTTHUMP00000219979
基因别名: AMACR; AMACRD; CBAS4; Da1-8; Macr1; Marc1; RACE; RM
UniProt ID: (Human) Q9UHK6, (Mouse) O09174
Entrez Gene ID: (Human) 23600, (Rat) 25284, (Mouse) 17117
