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Invitrogen
ANAPC2 Polyclonal Antibody
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ANAPC2 Antibody (PA5-143952) in ICC/IF
Immunocytochemistry analysis of ANAPC2 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in ANAPC2 Polyclonal antibody (Product # PA5-143952) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate... View More
产品信息
PA5-143952
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human ANAPC2 recombinant protein (Position: K51-R272).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HEK293 whole cell, human Jurkat whole cell, human HEPG2 whole cell, human HELA whole cell, monkey COS-7 whole cell, human A549 whole cell, human PC-3 whole cell, rat stomach tissue, rat testis tissue, rat lung tissue, rat PC-12 whole cell, mouse stomach tissue, mouse lung tissue, mouse RAW2647 whole cell. IHC: human rectal cancer tissue. ICC/IF: A431 cell. Flow: THP-1 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Anaphase-promoting complex subunit 2; APC2; Cyclosome subunit 2; expressed during mesenchymal induction 4; induced during mesenchymal induction 4
基因别名: 9230107K09Rik; AL024279; ANAPC2; APC2; Emi4; Imi4; KIAA1406; mKIAA1406
UniProt ID: (Human) Q9UJX6, (Mouse) Q8BZQ7
Entrez Gene ID: (Human) 29882, (Mouse) 99152
mitotic nuclear division
anaphase-promoting complex-dependent catabolic process
positive regulation of synaptic plasticity
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of axon extension
positive regulation of dendrite morphogenesis
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
protein K11-linked ubiquitination
positive regulation of synapse maturation
ubiquitin-dependent protein catabolic process
cell cycle
nervous system development
cell differentiation
