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Agrisera
AOX2/Plant alternative oxidase 2 Polyclonal Antibody
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AOX2/Plant alternative oxidase 2 Antibody (AS21-4532) in WB
Samples: Total protein 1-4 Cytoplasmic fraction 5-8 Nuclear fraction 9-12 20 µg/well of total protein extracted freshly from fractionation of Arabidopsis thaliana 7-day-old green seedlings and denatured with 2 x LaemmLi Sample Buffer at 90°C for 5 min were separated on 12% SDS-PAGE and blotted 1 h to nitrocellulose (pore size of 0.45 um), using wet transfer. Blot was blocked with 5% milk for 1 h/RT with agi... View More
产品信息
AS21-4532
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AOX2 protein sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
AOX2, also called alternative oxidase 2, is a mitochondrial inner membrane protein in plants that forms part of the cyanide resistant respiratory pathway. AOX2 bypasses complexes III and IV by oxidizing ubiquinol and reducing oxygen directly to water without proton pumping, thereby lowering ATP yield but limiting reactive oxygen species formation and maintaining redox balance when the cytochrome pathway is constrained. AOX2 expression is induced by stress signals, high reducing pressure in the ubiquinone pool, and metabolites such as pyruvate, which can allosterically activate the enzyme. Through flexible electron flow, AOX2 supports tolerance to cold, drought, pathogen challenge, and photorespiratory load, and helps stabilize metabolic fluxes and signaling between mitochondria and chloroplasts. In species with multiple AOX genes, AOX2 often shows tissue or developmental specificity, contributing to fine tuning of respiratory efficiency and stress resilience.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ALTERNATIVE OXIDASE; alternative oxidase 2; AT5G64210; MSJ1.5; MSJ1_5
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