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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • AP2A2 Antibodies

          Invitrogen

          AP2A2 Polyclonal Antibody

          查看其他9个AP2A2抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite AP2A2 Polyclonal Antibody

          • 抗体检测数据 (8)
          AP2A2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          AP2A2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 8

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          AP2A2 Antibody (PA5-143990) in ICC/IF

          Immunocytochemistry analysis of AP2A2 in PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in AP2A2 Polyclonal antibody (Product # PA5-143990) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          查看产品

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          AP2A2 Antibody in Immunocytochemistry (ICC/IF)
          AP2A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AP2A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AP2A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AP2A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AP2A2 Antibody in Western Blot (WB)
          AP2A2 Antibody in Flow Cytometry (Flow)
          AP2A2 Antibody in Flow Cytometry (Flow)
          AP2A2 Polyclonal Antibody

          产品信息

          PA5-143990

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          0.1-0.25 µg/mL
          -

          免疫组化(石蜡) (IHC (P))

          2-5 µg/mL
          -

          免疫细胞化学 (ICC/IF)

          5 µg/mL
          -

          流式细胞分析 (Flow)

          1-3 µg/1x10^6 cells
          -

          酶联免疫吸附实验 (ELISA)

          0.1-0.5 µg/mL
          -
          产品规格

          种属反应

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          E.coli-derived human AP2A2 recombinant protein (Position: D686-K825).
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Lyophilized

          浓度

          500 µg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS with 4mg trehalose

          内含物

          no preservative

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3075204

          产品详细信息

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human HepG2 whole cell, human HEK293 whole cell, human PC-3 whole cell, human SH-SY5Y whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue. IHC: human lymphoma tissue, human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue. ICC/IF: PC-3 cell. Flow: 293T cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          靶标信息

          Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Alpha adaptin is a subunit of the plasma membrane adaptor. It binds polyphosphoinositides.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: 100 kDa coated vesicle protein C; adapter-related protein complex 2 alpha-2 subunit; adapter-related protein complex 2 subunit alpha-2; adaptin alpha A; Adaptin alpha B; adaptin, alpha B; Adaptor protein complex AP-2 subunit alpha-2; adaptor protein complex AP-2, alpha 2 subunit; adaptor related protein complex 2, alpha 2 subunit; Adaptor-related protein complex 2 subunit alpha-2; adaptor-related protein complex 2, alpha 2 subunit; adaptor-related protein complex AP-2, alpha 2 subunit; ADTAA; Alpha adaptin A; Alpha adaptin C; Alpha-adaptin C; alpha-adaptin C; Huntingtin interacting protein J; alpha-C adaptin; Alpha1 adaptin; Alpha2 adaptin; Alpha2-adaptin; AP-2 complex subunit alpha-2; AP2 alpha; AP2A1; CLAPA1; Clathrin assembly; Clathrin assembly protein complex 2 alpha-C large chain; clathrin-associated/assembly/adaptor protein, large, alpha 2; HIP 9; HIP-9; Huntingtin yeast partner J; Huntingtin-interacting protein 9; Huntingtin-interacting protein J; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

          查看更多 收起

          基因别名: 2410074K14Rik; ADTAB; AF006990; AP2A2; AW146353; C78001; CLAPA2; HIP-9; HIP9; HYPJ; KIAA0899; L25

          查看更多 收起

          UniProt ID: (Human) O94973, (Mouse) P17427

          查看更多 收起

          Entrez Gene ID: (Human) 161, (Rat) 81637, (Mouse) 11772

          查看更多 收起

          功能
          molecular_function protein binding lipid binding protein transporter activity protein kinase binding protein complex binding clathrin adaptor activity membrane traffic protein
          参与通路
          intracellular protein transport microtubule-based movement antigen processing and presentation of exogenous peptide antigen via MHC class II negative regulation of epidermal growth factor receptor signaling pathway ephrin receptor signaling pathway regulation of defense response to virus by virus Wnt signaling pathway, planar cell polarity pathway clathrin-mediated endocytosis endocytosis biological_process vesicle-mediated transport transport protein transport regulation of hematopoietic stem cell differentiation
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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