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Invitrogen
AP2M1 Monoclonal Antibody (OTI1F10)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 7
AP2M1 Antibody (MA5-25570) in ICC/IF
Immunofluorescence analysis of AP2M1 was performed using Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with AP2M1 Mouse Monoclonal Antibody (Product # MA5-25570) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 ... View More
产品信息
MA5-25570
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
OTI1F10
抗原
Human recombinant protein fragment corresponding to amino acids 97-383 of AP2M1 produced in E.coli
偶联物
形式
Liquid
浓度
1.00 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 1% BSA, 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2722871
靶标信息
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: adapter-related protein complex 2 mu subunit; adapter-related protein complex 2 subunit mu; Adaptin-mu2; Adaptor protein complex AP-2 subunit mu; adaptor related protein complex 2, mu 1 subunit; Adaptor-related protein complex 2 subunit mu; adaptor-related protein complex 2, mu 1 subunit; AP-2 complex subunit mu; AP-2 mu 2 chain; AP-2 mu chain; clathrin adaptor complex AP2, mu subunit; clathrin assembly protein complex 2 medium chain; Clathrin assembly protein complex 2 mu medium chain; clathrin coat adaptor protein AP50; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; clathrin-associated/assembly/adaptor protein, medium 1; coat assembly protein complex 50 kD; HA2 50 kDA subunit; Mu2-adaptin; Plasma membrane adaptor AP-2 50 kDa protein; plasma membrane adaptor AP-2 50kDA protein
基因别名: AP2M1; AP50; CLAPM1; KIAA0109; mu2
UniProt ID: (Human) Q96CW1, (Rat) P84092, (Mouse) P84091
Entrez Gene ID: (Human) 1173, (Rat) 116563, (Mouse) 11773
intracellular protein transport
microtubule-based movement
antigen processing and presentation of exogenous peptide antigen via MHC class II
negative regulation of epidermal growth factor receptor signaling pathway
ephrin receptor signaling pathway
regulation of defense response to virus by virus
Wnt signaling pathway, planar cell polarity pathway
clathrin-mediated endocytosis
negative regulation of protein localization to plasma membrane
endocytosis
vesicle-mediated transport
