Invitrogen

APOE Monoclonal Antibody (GT1627)

查看其他34个APOE抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (2)

APOE Antibody in ELISA (ELISA)

APOE Antibody in ELISA (ELISA)

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APOE Antibody (MA5-50301) in ELISA

Sandwich ELISA of APOE in recombinant full-length human apolipoprotein E. Samples were incubated with APOE monoclonal antibody (Product # MA5-50301) using a dilution of 5 µg/mL (capture) and APOE polyclonal antibody (Product # PA5-27088) as detection antibody at concentration of 1 µg/mL. Followed by Rabbit IgG antibody (HRP) at a dilution of 1:10,000.

APOE Antibody in ELISA (ELISA)

APOE Antibody in ELISA (ELISA)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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APOE Antibody in ELISA (ELISA) — Sandwich ELISA of APOE in recombinant full-length human apolipoprotein E. Samples were incubated with APOE monoclonal antibody (Product # MA5-50301) using a dilution of 5 µg/mL (capture) and APOE polyclonal antibody (Product # PA5-27088) as detection antibody at concentration of 1 µg/mL. Followed by Rabbit IgG antibody (HRP) at a dilution of 1:10,000.

APOE Antibody in ELISA (ELISA) — Indirect ELISA of APOE in recombinant full-length human apolipoprotein E (400-6.25 ng/mL). Samples were probed with APOE monoclonal antibody (Product # MA5-50301) at a dilution of 1 µg/mL followed by Mouse IgG antibody (HRP) using a dilution of 1:10,000.

产品信息

MA5-50301

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

免疫沉淀 (IP)

Assay-dependent

-

产品规格

种属反应

Human, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

GT1627

抗原

Recombinant protein encompassing a sequence within the center region of human Apolipoprotein E. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein A

保存液

PBS with 20% glycerol

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

This product can be used in Sandwich ELISA to detect APOE using MA5-50301 as a capture antibody and PA5-27088 as the detection antibody.

靶标信息

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Apo-E; ApoE4; Apolipoprotein; Apolipoprotein E; apolipoprotein E4; Apoprotein; B2G1; BG; MGC1571

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基因别名: APOE; APOEA

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UniProt ID: (Rat) P02650

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Entrez Gene ID: (Rat) 25728

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beta-amyloid binding receptor binding lipid transporter activity phospholipid binding heparin binding lipid binding cholesterol binding antioxidant activity cholesterol transporter activity identical protein binding protein homodimerization activity hydroxyapatite binding metal chelating activity tau protein binding low-density lipoprotein particle receptor binding phosphatidylcholine-sterol O-acyltransferase activator activity very-low-density lipoprotein particle receptor binding lipoprotein particle binding apolipoprotein

negative regulation of endothelial cell proliferation response to dietary excess lipid metabolic process triglyceride metabolic process cholesterol biosynthetic process cholesterol catabolic process lipid transport cellular calcium ion homeostasis receptor-mediated endocytosis response to oxidative stress G-protein coupled receptor signaling pathway nitric oxide mediated signal transduction aging protein localization cholesterol metabolic process regulation of gene expression negative regulation of platelet activation positive regulation of cholesterol esterification positive regulation of cholesterol efflux peripheral nervous system axon regeneration long-chain fatty acid transport protein import triglyceride catabolic process cGMP-mediated signaling negative regulation of blood coagulation positive regulation of cGMP biosynthetic process neuron projection regeneration axon regeneration regulation of cholesterol transport regulation of Cdc42 protein signal transduction response to retinoic acid positive regulation of low-density lipoprotein particle receptor catabolic process response to insulin cholesterol efflux phospholipid efflux very-low-density lipoprotein particle remodeling low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly chylomicron remnant clearance high-density lipoprotein particle clearance very-low-density lipoprotein particle clearance lipoprotein metabolic process lipoprotein biosynthetic process lipoprotein catabolic process vasodilation cholesterol homeostasis positive regulation of catalytic activity negative regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of blood vessel endothelial cell migration reverse cholesterol transport response to ethanol negative regulation of cholesterol biosynthetic process positive regulation of axon extension positive regulation of lipid biosynthetic process oligodendrocyte differentiation artery morphogenesis negative regulation of inflammatory response positive regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis negative regulation of lipid biosynthetic process maintenance of location in cell lipid homeostasis fatty acid homeostasis positive regulation of dendritic spine development negative regulation of dendritic spine development cellular response to interleukin-1 cellular response to growth factor stimulus cellular response to cholesterol cardiovascular system development negative regulation of cholesterol efflux AMPA glutamate receptor clustering NMDA glutamate receptor clustering regulation of beta-amyloid clearance regulation of neuron death negative regulation of neuron death positive regulation of neuron death negative regulation of postsynaptic membrane organization positive regulation of postsynaptic membrane organization negative regulation of presynaptic membrane organization positive regulation of presynaptic membrane organization positive regulation of beta-amyloid formation negative regulation of beta-amyloid formation regulation of tau-protein kinase activity negative regulation of dendritic spine maintenance positive regulation of dendritic spine maintenance positive regulation of phospholipid efflux positive regulation of neurofibrillary tangle assembly negative regulation of phospholipid efflux negative regulation of lipid transport across blood brain barrier positive regulation of lipid transport across blood brain barrier