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Invitrogen
ATF Polyclonal Antibody
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FIGURE: 1 / 1
ATF Antibody (PA5-81146) in ICC/IF
Immunofluorescence staining of ATF in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ATF Polyclonal Antibody (Product # PA5-81146, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.
Product Details
PA5-81146
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human ATF2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human ATF2.
Target Information
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating 2; Activating transcription factor 2; ATF2; bZIP transcription factor; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB-2; cyclic AMP response element-binding protein (HB16); Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354; unnamed protein product
Gene Aliases: ATF2; CRE-BP1; CREB-2; CREB2; CREBP1; HB16; TREB7
UniProt ID: (Human) P15336
Entrez Gene ID: (Human) 1386
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
histone acetyltransferase activity
protein binding
cAMP response element binding protein binding
zinc ion binding
H4 histone acetyltransferase activity
protein kinase binding
cAMP response element binding
identical protein binding
protein homodimerization activity
leucine zipper domain binding
sequence-specific DNA binding
histone H2B acetyltransferase activity
metal ion binding
protein heterodimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
in utero embryonic development
NK T cell differentiation
liver development
hematopoietic progenitor cell differentiation
outflow tract morphogenesis
brainstem development
growth plate cartilage chondrocyte differentiation
growth plate cartilage chondrocyte proliferation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein import into nucleus
lipid metabolic process
apoptotic process
response to osmotic stress
cellular response to DNA damage stimulus
vacuole organization
JNK cascade
heart development
gene expression
positive regulation of gene expression
negative regulation of angiogenesis
peptidyl-threonine phosphorylation
abducens nucleus development
hypoglossal nucleus development
facial nucleus development
BMP signaling pathway
intra-S DNA damage checkpoint
positive regulation of transforming growth factor beta2 production
cellular response to oxidative stress
p38MAPK cascade
mRNA transcription from RNA polymerase II promoter
positive regulation of gene expression, epigenetic
positive regulation of transcription from RNA polymerase II promoter
white fat cell differentiation
positive regulation of sequence-specific DNA binding transcription factor activity
neurofilament cytoskeleton organization
detection of cell density
adipose tissue development
cellular response to anisomycin
motor neuron apoptotic process
hepatocyte apoptotic process
cellular response to virus
positive regulation of cardiac muscle myoblast proliferation
positive regulation of mitochondrial membrane permeability involved in apoptotic process
apoptotic process involved in development
intrinsic apoptotic signaling pathway in response to hypoxia
cellular response to leucine starvation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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