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Invitrogen
ATF2 Recombinant Rabbit Monoclonal Antibody (SZ17-01)
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ATF2 Antibody (MA5-32022) in ICC/IF
Immunofluorescence analysis of Cyclic AMP-dependent transcription factor ATF-2 was performed using 70% confluent log phase FaDu cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ATF2 Recombinant Rabbit Monoclonal Antibody (SZ17-01) (Product # MA5-32022) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adso... View More
产品信息
MA5-32022
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SZ17-01
抗原
Synthetic peptide within Human ATF2 aa 456-505
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Activating 2; Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB-2; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; MXBP protein; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354
基因别名: Atf-2; ATF2; CRE-BP; CRE-BP1; CREB-2; CREB2; CREBP1; D130078H02Rik; D18875; HB16; mXBP; Tg(Gzma-Klra1)7Wum; TREB7
UniProt ID: (Human) P15336, (Mouse) P16951
Entrez Gene ID: (Human) 1386, (Mouse) 11909
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
histone acetyltransferase activity
protein binding
cAMP response element binding protein binding
protein kinase binding
cAMP response element binding
metal ion binding
protein heterodimerization activity
nucleic acid binding
DNA binding
transferase activity
sequence-specific DNA binding
basic leucine zipper transcription factor
negative regulation of transcription from RNA polymerase II promoter
outflow tract morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
response to osmotic stress
cellular response to DNA damage stimulus
response to water deprivation
histone acetylation
intra-S DNA damage checkpoint
positive regulation of transforming growth factor beta2 production
positive regulation of neuron apoptotic process
fat cell differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of epithelial cell proliferation
regulation of sequence-specific DNA binding transcription factor activity
positive regulation of sequence-specific DNA binding transcription factor activity
adipose tissue development
amelogenesis
positive regulation of mitochondrial membrane permeability involved in apoptotic process
transcription, DNA-templated
