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Invitrogen
ATF5 Recombinant Rabbit Monoclonal Antibody (SD2099)
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ATF5 Antibody (MA5-32365) in ICC/IF
Immunocytochemistry analysis of ATF5 in RAW264.7 cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with ATF5 monoclonal antibody (Product # MA5-32365) using a dilution of 1:100 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of ... View More
产品信息
MA5-32365
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SD2099
抗原
Synthetic peptide within Human ATF5 aa 203-252
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
ATF5 is a protein coding gene. Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate. Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation. Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes. May be involved in osteogenic differentiation. Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1. Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent. Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver. In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase. May act as a negative regulator of IL1B transduction pathway in liver. Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members. Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation. Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Activating transcription factor 5; Activating transcription factor 5-alpha/beta; activating transcription factor 7; activating transcription factor X; BZIP protein ATF7; cAMP-dependent transcription factor ATF-5; Cyclic AMP-dependent transcription factor ATF-5; FLJ34666; NAP1; NRIF3-associated protein; Transcription factor ATFx; Transcription factor-like protein ODA-10
基因别名: AFTA; ATF5; Atf7; ATFX; HMFN0395; Nap1; ODA-10
UniProt ID: (Human) Q9Y2D1, (Rat) Q6P788, (Mouse) O70191
Entrez Gene ID: (Human) 22809, (Rat) 282840, (Mouse) 107503
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
tubulin binding
kinase binding
heat shock protein binding
sequence-specific DNA binding
transcription regulatory region DNA binding
DNA binding
DNA-binding transcription factor
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
circadian rhythm
negative regulation of cell proliferation
post-embryonic development
olfactory bulb interneuron development
cerebellar granule cell precursor proliferation
multicellular organism growth
negative regulation of apoptotic process
fat cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of centrosome cycle
negative regulation of astrocyte differentiation
negative regulation of cell cycle G2/M phase transition
transcription, DNA-templated
regulation of gene expression
olfactory bulb interneuron differentiation
olfactory lobe development
regulation of cell proliferation
negative regulation of neurogenesis
regulation of cell cycle
