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Agrisera
ATG4/Autophagy protein 4 (algal) Polyclonal Antibody
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ATG4/Autophagy protein 4 (algal) Antibody (AS15-2831) in WB
10 ng of recombinant, purified Chlamydomonas reinhardtii ATG 4 (left) and 15 µg of Chlamydomonas reinhardtii total cell extract (right) were separated on 12% SDS-PAGE and blotted 1 h to nitrocellulose membrane using semi-dry transfer. Blots were blocked with 5% dry milk in PBS for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5,000 over night at 4... View More
产品信息
AS15-2831
产品规格
种属反应
Algae
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant ATG4 of Chlamydomonas reinhardtii.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 0.5 mL of sterile water.
Specific Species Reactivity: Chlamydomonas reinhardtii, Chlorococcum dorsiventrale
靶标信息
ATG4 is a cysteine protease central to the autophagy pathway in plants. It processes ATG8 by cleaving a C terminal extension to expose a glycine required for conjugation to phosphatidylethanolamine, enabling formation and maturation of autophagosomes. ATG4 also deconjugates ATG8 from membranes, allowing recycling and fine tuning of autophagic flux. Plant ATG4 activity is modulated by redox status and interacts with regulatory factors to adjust autophagy during nutrient limitation, senescence, and stress such as drought, salinity, and pathogen challenge. Multiple ATG4 isoforms can provide substrate specificity for different ATG8 variants, influencing selective autophagy of organelles and protein aggregates. Genetic studies in Arabidopsis and crops link ATG4 to growth maintenance, stress tolerance, and nutrient remobilization by sustaining efficient cellular recycling.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AtAPG4a; AtAPG4b; AtATG4a; AtATG4b
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