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ATG8/Autophagy-related protein Polyclonal Antibody
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ATG8/Autophagy-related protein Antibody (AS14-2769) in WB
30 µg of total protein from Chlamydomonas reinhardtii, control (C), autophagy induced (A), extracted with lysis buffer according to Perez-Perez et al. 2010 (Plant Physiology 152:1,874-1,888) were separated on 15% SDS-PAGE and blotted 1 h to nitrocellulose membrane using semi-dry or tank transfer. Blots were blocked with 5% milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the prima... View More
产品信息
AS14-2769
产品规格
种属反应
Algae,
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Fragment of recombinant ATG8 from Chlamydomonas reinhardtii.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Aponogeton madagascariensis, Chlamydononas reinhardtii, Chlorococcum dorsiventrale, Haematococcus lacustris, Nicotiana benthamiana, Populus trichocarpa, Solanum lycopersicum, Zea mays
靶标信息
Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Apg8 is required for autophagy (intracellular bulk protein degradation) in yeast. Starved yeast cells take up their own cytoplasm into vacuoles through autophagic bodies. Autophagic bodies form a double-membraned structure called the autophagosome, which subsequently fuses with the vacuole/lysosome. This process similar in mammals. Two sets of genes, APG and AUT, have been identified with this process, and are responsible for two ubiquitin-like systems Apg12 and Apg8, respectively. Apg12 is synthesized in its mature form and seems to have one target, Apg5. Almost all Apg12 molecules are conjugated with Apg5.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: APG8; AUT7; CVT5
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