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Invitrogen
ATP Citrate Lyase Polyclonal Antibody
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ATP Citrate Lyase Antibody (PA5-78712) in ICC/IF
Immunocytochemistry analysis of ACLY using anti-ACLY antibody (Product # PA5-78712) . ACLY was detected in an immunocytochemical section of A594 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-ACLY antibody (Product # PA5-78712) overnight at 4°C. DyLight®488 Conjugated... View More
产品信息
PA5-78712
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human MOLT4 whole cell, human U87 whole cell, human U251 whole cell, human 293T whole cell, human Hela whole cell, human T47D whole cell, human HepG2 whole cell, rat pancreas tissue, mouse NIH/3T3 whole cell. IHC: human colonic adenocarcinoma tissue, human hepatocellular carcinoma tissue, human laryngeal squamous cell carcinoma tissue, human placenta tissue, human colon cancer tissue, mouse testis tissue, rat testis tissue. ICC/IF: A594 cell. Flow: HepG2 cell.
靶标信息
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ACL; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; Citrate cleavage enzyme
基因别名: A730098H14Rik; ACL; ACLY; ATPCL; AW538652; CLATP
UniProt ID: (Human) P53396, (Mouse) Q91V92, (Rat) P16638
Entrez Gene ID: (Human) 47, (Mouse) 104112, (Rat) 24159
ATP citrate synthase activity
protein binding
ATP binding
metal ion binding
cofactor binding
nucleotide binding
catalytic activity
succinate-CoA ligase (ADP-forming) activity
transferase activity
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
lyase activity
transferase
acetyl-CoA biosynthetic process
citrate metabolic process
oxaloacetate metabolic process
fatty acid biosynthetic process
cholesterol biosynthetic process
lipid biosynthetic process
positive regulation of cellular metabolic process
long-chain fatty-acyl-CoA biosynthetic process
acetyl-CoA metabolic process
lipid metabolic process
metabolic process
cellular carbohydrate metabolic process
