Invitrogen

ATP1A3 Monoclonal Antibody (XVIF9-G10)

Name: ATP1A3 Monoclonal Antibody (XVIF9-G10)
Brand: Invitrogen
SKU: MA3-915

高级验证

45个已发表图片

109 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

ATP1A3 Antibody in Immunocytochemistry (ICC/IF)

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ATP1A3 Antibody (MA3-915) in ICC/IF

Immunofluorescence analysis of ATP1A3 was performed using SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ATP1A3 Monoclonal Antibody (XVIF9-G10) (Product # MA3-915) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000), for ... View More

ATP1A3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP1A3 Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

ATP1A3 Antibody in Immunohistochemistry (IHC)

ATP1A3 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

MA3-915

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看67篇已发表文章 67篇已发表文章

免疫组化 (IHC)

查看23篇已发表文章 23篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫组化（冰冻） (IHC (F))

3 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

查看13篇已发表文章 13篇已发表文章

流式细胞分析 (Flow)

1 µg / 10^6 cells

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

免疫电镜技术 (IM)

查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

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其他PubMed (Misc)

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产品规格

种属反应

Bovine, Dog, Guinea pig, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat

已发表种属

Amphibian, Bovine, C. elegans, Gerbil, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat, Shark

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

XVIF9-G10

抗原

Canine cardiac microsomes.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2274447

产品详细信息

MA3-915 detects sodium/potassium ATPase from human, monkey, bovine, sheep, canine, rabbit, guinea pig, mouse and rat tissue. This antibody is specific for the alpha-3 subunit.

MA3-915 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects an ~110 kDa protein representing the alpha-3 subunit of the sodium/potassium ATPase from canine skeletal muscle extract. Immunohistochemical staining of sodium/potassium ATPase in rat retina with MA3-915 yields a pattern consistent with plasma membrane localization.

The MA3-915 antigen is canine cardiac microsomes.

靶标信息

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ATPase, Na+/K+ transporting, alpha 3 polypeptide; ATPase, Na+K+ transporting, alpha 3 subunit; EC 3.6.3.9; Na(+)/K(+) ATPase alpha(III) subunit; Na(+)/K(+) ATPase alpha-3 subunit; Na+, K+ activated adenosine triphosphatase alpha subunit; Na+/K+ -ATPase alpha 3 subunit; Na+/K+ ATPase 3; Sodium Potassium ATPase; Sodium pump 3; Sodium pump subunit alpha-3; sodium-potassium ATPase catalytic subunit alpha-3; sodium-potassium-ATPase, alpha 3 polypeptide; sodium/potassium-transporting ATPase alpha-3 chain; Sodium/potassium-transporting ATPase subunit alpha-3

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基因别名: AHC2; ATP1A1; ATP1A3; Atpa-2; ATPa1a3; CAPOS; DYT12; RDP

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UniProt ID: (Human) P13637, (Rat) P06687, (Mouse) Q6PIC6