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Agrisera
AUX1/Auxin transporter protein 1 Polyclonal Antibody
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AUX1/Auxin transporter protein 1 Antibody (AS16-3159) in WB
Arabidopsis thaliana AUX 1 was expressed in insect cells using baculovirus infection. Expression was driven by the strong polyhedron promoter. Cultures of Sf 9 cells (20 mL, approx. 2 x 107 cells) were infected with virus at multiplicities of infection (MOI) of 0.1,1 and 2.5.2 m L samples were harvested by centrifugation after 3 days, lysed (20 m M Tris/HCl, p H 7.4,200 m M Na Cl, 1 m M EDTA, 1% Tween 20, p... View More
产品信息
AS16-3159
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana AUX1.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana (recombinant AUX1)
靶标信息
Arabidopsis thaliana AUX1 encodes a polytopic membrane transporter of the AUX/LAX (AAAP superfamily) auxin-import system that localizes predominantly to the plasma membrane in a cell type- and polarity-dependent manner (notably at the apical plasma membrane of protophloem cells) and also traffics through dynamic subpopulations of Golgi/endosomal compartments. The AUX1 protein is a multi-pass integral membrane carrier with an experimentally supported topology of 11 transmembrane segments, with an intracellular N-terminus and extracellular C-terminus, consistent with its role as a selective importer of the major natural auxin indole-3-acetic acid. Functionally, AUX1 operates as a high-affinity auxin influx carrier that helps establish and maintain local auxin maxima and directional auxin flow in concert with efflux systems, thereby supporting multiple auxin-dependent developmental programs; classic loss-of-function phenotypes include impaired root gravitropism and defects in lateral root initiation/positioning, reflecting AUX1's role in regulating auxin availability in outer root tissues (e.g., lateral root cap and epidermis) that coordinate organ patterning and growth responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AtAUX1; AUXIN RESISTANT 1; F16M14.5; F16M14_5; MAP1; MODIFIER OF ARF7/NPH4 PHENOTYPES 1; PIR1; WAV5; WAVY ROOTS 5
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