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Rockland
Abi1 Polyclonal Antibody
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Abi1 Antibody (600-401-GT5) in WB
Western Blot of Rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: MEF3 KO. Lane 2: MEF3 WT. Lane 3: Mouse brain. Lane 4: Rat brain. Lane 5: HCT-116 WCL. Lane 6: HeLa WCL. Lane 7: NIH/3T3 WCL. Load: 35 µg per lane. Primary antibody: Abi1 antibody at 1:1,000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.
产品信息
600-401-GT5
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to near c-terminal of the mouse Abi-1 protein.
偶联物
形式
Liquid
浓度
1.07 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Abi1 is directed against mouse Abi1. A BLAST analysis was used to suggest cross reactivity with this protein in human and rat based on 100% homology for the immunogen sequence. Reactivity against homologues from other sources is not known.
靶标信息
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Abelson interactor 1; Abi-1; Abl interactor 1; Ablphilin-1; e3B1; EL2; eps8 binding protein; Eps8 SH3 domain-binding protein; Eps8-binding protein; HPP; HS3; spectrin SH3 domain binding protein 1; Spectrin SH3 domain-binding protein 1; SPH3; SPTA
基因别名: Abi1; E3B1; NAP1; Ssh3bp1
UniProt ID: (Mouse) Q8CBW3
Entrez Gene ID: (Mouse) 11308
