Invitrogen

Adenosine Deaminase Polyclonal Antibody

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问题与答案

问题与答案

问题与答案

抗体检测数据 (4)

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Adenosine Deaminase Antibody (PA5-78722) in IHC (P)

Immunohistochemistry analysis of ADA on paraffin-embedded mouse intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with ADA polyclonal antibody (Product# PA5-78722) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Developm... View More

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Adenosine Deaminase Antibody in Western Blot (WB)

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Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ADA on paraffin-embedded mouse intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with ADA polyclonal antibody (Product# PA5-78722) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ADA on paraffin-embedded rat spleen tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with ADA polyclonal antibody (Product# PA5-78722) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ADA on paraffin-embedded rat brain tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with ADA polyclonal antibody (Product# PA5-78722) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Adenosine Deaminase Antibody in Western Blot (WB) — Western blot analysis of ADA using Adenosine Deaminase Polyclonal Antibody (Product # PA5-78722). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: rat brain tissue lysates. Lane 2: rat liver tissue lysates. Lane 3: rat liver tissue lysates. Lane 4: mouse liver tissue lysates. Lane 5: mouse spleen tissue lysates. Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with Adenosine Deaminase Polyclonal Antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADA at approximately 41 kDa. The expected band size for ADA is at 41 kDa.

Immunogen image

Adenosine Deaminase Polyclonal Antibody

Immunogen Image Adenosine Deaminase Polyclonal Antibody

产品信息

PA5-78722

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

-

酶联免疫吸附实验 (ELISA)

1-5 µg/mL (capture)

-

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived rat ADA recombinant protein (Position: A2-H241).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: rat brain tissue, rat liver tissue, rat liver tissue, mouse liver tissue, mouse spleen tissue, mouse NIH/3T3 whole cell. IHC: rat spleen tissue, mouse intestine tissue, rat brain tissue.

靶标信息

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. May act as a positive regulator of T-cell coactivation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Adenosine aminohydrolase; Adenosine deaminase; RP11-61L14.5

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基因别名: Ada

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UniProt ID: (Mouse) P03958, (Rat) Q920P6

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Entrez Gene ID: (Mouse) 11486, (Rat) 24165

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purine nucleoside binding adenosine deaminase activity zinc ion binding hydrolase activity deaminase activity metal ion binding deaminase

response to hypoxia in utero embryonic development histamine secretion trophectodermal cell differentiation liver development placenta development germinal center B cell differentiation positive regulation of germinal center formation negative regulation of leukocyte migration negative regulation of mature B cell apoptotic process adenosine catabolic process deoxyadenosine catabolic process cell adhesion nucleotide metabolic process purine ribonucleoside monophosphate biosynthetic process positive regulation of heart rate lung development positive regulation of B cell proliferation purine nucleotide salvage positive regulation of T cell differentiation in thymus response to vitamin E regulation of cell-cell adhesion mediated by integrin T cell activation negative regulation of circadian sleep/wake cycle, non-REM sleep negative regulation of apoptotic process hypoxanthine salvage response to morphine regulation of T cell differentiation positive regulation of T cell differentiation positive regulation of smooth muscle contraction dATP catabolic process adenosine metabolic process hypoxanthine biosynthetic process inosine biosynthetic process xanthine biosynthetic process positive regulation of alpha-beta T cell differentiation lung alveolus development Peyer's patch development embryonic digestive tract development negative regulation of inflammatory response positive regulation of calcium-mediated signaling positive regulation of T cell receptor signaling pathway positive regulation of T cell activation negative regulation of adenosine receptor signaling pathway negative regulation of penile erection negative regulation of thymocyte apoptotic process negative regulation of mucus secretion aging response to drug response to hydrogen peroxide regulation of circadian sleep/wake cycle, sleep