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Invitrogen
African Swine Fever Virus Structural Protein p54 Recombinant Rabbit Monoclonal Antibody (HL1219)
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African Swine Fever Virus Structural Protein p54 Antibody (MA5-50305) in ICC/IF
Immunocytochemical analysis of African Swine Fever Virus Structural Protein p54 using African Swine Fever Virus Structural Protein p54 Recombinant Monoclonal Antibody (Product # MA5-50305) in Mock and transfected 293T cells at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASFV p54 stained by ASFV p54 antibody (MA5-50305) diluted at 1:500. Blue: Fluoroshield with DAPI.
产品信息
MA5-50305
应用
建议稀释比
已发表文章
产品规格
种属反应
Virus
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
HL1219
抗原
Recombinant protein encompassing a sequence within the center region of ASFV p54. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This product can be used in Sandwich ELISA to detect African Swine Fever Virus Structural Protein p54 using MA5-50304 as a capture antibody and MA5-50305 as the detection antibody.
靶标信息
The African Swine Fever Virus (ASFV) structural protein p54, encoded by the E183L gene, is a crucial component of the virus's architecture, playing a significant role in its morphogenesis. This 25-kDa polypeptide is essential for the recruitment of envelope precursors to assembly sites, contributing to the formation of viral particles. It is highly antigenic and has been localized within the replication factories of infected cells, predominantly associated with membranes and immature virus particles. The protein p54 has been shown to interact with target cells, aiding in the virus's attachment and entry, alongside other viral proteins like p30. Attempts to inactivate the p54 gene have demonstrated its necessity for ASFV replication, underscoring its potential as a target for therapeutic interventions. Its structural and functional properties make p54 a focal point of study in the development of ASFV treatments and prevention strategies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: African swine fever virus p54
