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Invitrogen
Argonaute 3 Recombinant Rabbit Monoclonal Antibody (22H29L11)
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Argonaute 3 Antibody (702721) in WB
Western blot was performed using Anti-Argonaute 3 Recombinant Rabbit Monoclonal Antibody (Product # 702721) and a 97 kDa band corresponding to Argonaute 3 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of T-47D (Lane 1), MCF7 (Lane 2), HeLa (Lane 3), Caco-2 (Lane 4), HEK-293 (Lane 5), Hep G2 (Lane 6) and HCT 116 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
702721
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
22H29L11
抗原
Peptides corresponding to human Argonaute 3 [1) aa10-aa29 2) aa385-aa404]
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Mouse, Rat, Bovine.
靶标信息
AGO3 (protein argonaute-3) is required to RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. AGO3 is proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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