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Rockland
Aspartate Transaminase Polyclonal Antibody, HRP
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Aspartate Transaminase Antibody (200-603-144-0100) in DB
Dot Blot of Sheep anti-Aspartate Transaminase Antibody. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: Sh-a-Aspartate Transaminase (AST) HRP 200-603-144 at 1µg/mL. Blocking Buffer: BlockOut MB-073 for 30 min at RT.
产品信息
200-603-144-0100
应用
建议稀释比
已发表文章
产品规格
种属反应
Pig
宿主/亚型
Sheep
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Aspartate Aminotransferase (AST) [Pig Heart]
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl
内含物
0.01% gentamicin sulfate
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart]. Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined.
靶标信息
GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Aspartate aminotransferase, cytoplasmic; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; Glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); Transaminase A
基因别名: CASPAT; cCAT; GOT1
UniProt ID: (Pig) P00503
Entrez Gene ID: (Pig) 396967
2-oxoglutarate metabolic process
oxaloacetate metabolic process
glycerol biosynthetic process
aspartate metabolic process
aspartate catabolic process
glutamate metabolic process
Notch signaling pathway
glutamate catabolic process to aspartate
glutamate catabolic process to 2-oxoglutarate
cellular response to insulin stimulus
response to glucocorticoid
fatty acid homeostasis
