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Invitrogen
Ataxin 7 Polyclonal Antibody
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Ataxin 7 Antibody (PA1-749) in ICC/IF
Immunofluorescence analysis of Ataxin 7 was performed using 70% confluent log phase U-87 MG cells treated with 100 ng of Nocodazole for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ataxin 7 Rabbit Polyclonal Antibody (Product # PA1-749) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Seco... View More
产品信息
PA1-749
应用
建议稀释比
已发表文章
产品规格
种属反应
Fruit fly,
Human
已发表种属
C. elegans,
Fruit fly,
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide: M(1) S E R A A D D V R G E P R R A A(17) C
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-749 detects ataxin 7 protein from transfected cell lysate.
PA1-749 has successfully been used in immunohistochemistry and Western blot procedures. By Western blot, this antibody detects an ~96 kDa protein representing human ataxin 7 in transfected COS-1 cells.
The PA1-749 immunizing peptide corresponds to amino acid residues 1-17 from human ataxin 7. This sequence is completely conserved in mouse ataxin 7. This peptide (Cat. # PEP-217) is available for use in neutralization and control experiments.
靶标信息
Spinocerebellar ataxia type 7 (SCA7) belongs to a class of hereditary neurodegenrative diseases characterized by abnormally expanded polyglutamine (CAG) repeats at the N-terminus. This autosomal dominant cerebellar ataxia primarily affects the cerebellum, retina, and brain stem and causes dementia, macular degeneration and other neurodegenerative characteristics. SCA7 gene encodes the ubiquitous 892 residue protein ataxin-7. This protein is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Ataxin-7; Atxn7-PA; Atxn7-PB; Atxn7-PC; Atxn7-PD; CG9866-PA; CG9866-PB; CG9866-PC; CG9866-PD; LD40170p; Spinocerebellar ataxia type 7 protein
基因别名: ADCAII; Ataxin7; ATXN7; CG9866; Dmel\CG9866; Dmel_CG9866; OPCA3; SCA7
UniProt ID: (Human) O15265
Entrez Gene ID: (Human) 6314, (Fruit fly) 33423
SAGA complex assembly
positive regulation of histone H2B ubiquitination
microtubule cytoskeleton organization
transcription, DNA-templated
nucleus organization
visual perception
histone deubiquitination
negative regulation of phosphorylation
negative regulation of insulin-like growth factor receptor signaling pathway
positive regulation of transcription from RNA polymerase II promoter
