Search
Invitrogen
Aurora B Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
Aurora B Antibody (PA5-85633) in WB
Western Blot using Aurora B Polyclonal Antibody (Product # PA5-85633). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora B Polyclonal Antibody (Product # PA5-85633) diluted at 1:5,000.
Product Details
PA5-85633
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Aurora B. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.81 mg/mL
Amount
81 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Pig (89%), Bovine (90%).
Positive Control: HeLa, HeLa (100 ng/ml Nocodazole, 24hr), 293T transfected with Aurora B.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: AIM-1; ARK-2; Aurora 1; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora- and IPL1-like midbody-associated protein 1; aurora- and Ipl1-like midbody-associated protein 1 homolog; aurora-1; aurora-B; aurora-related kinase 2; Aurora/IPL1-related kinase 2; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; STK-1; unnamed protein product
Gene Aliases: AIK2; AIM-1; AIM1; AIRK2; ARK-2; ARK2; AurB; AURKB; aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK-1; STK1; STK12; STK5
UniProt ID: (Human) Q96GD4
Entrez Gene ID: (Human) 9212
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
mitotic cytokinesis
negative regulation of B cell apoptotic process
spindle organization
mitotic spindle organization
positive regulation of microtubule depolymerization
positive regulation of telomere maintenance
regulation of cytokinesis
negative regulation of cytokinesis
positive regulation of cytokinesis
regulation of microtubule-based process
protein localization to kinetochore
cellular response to UV
cleavage furrow formation
post-translational protein modification
cell cycle G2/M phase transition
mitotic cytokinesis checkpoint
negative regulation of innate immune response
mitotic spindle midzone assembly
cell division
attachment of mitotic spindle microtubules to kinetochore
regulation of chromosome segregation
midbody abscission
positive regulation of mitotic sister chromatid segregation
positive regulation of cell cycle process
positive regulation of mitotic cell cycle spindle assembly checkpoint
mitotic spindle assembly
repair of mitotic kinetochore microtubule attachment defect
cGAS/STING signaling pathway
negative regulation of cGAS/STING signaling pathway
regulation of signal transduction by p53 class mediator
positive regulation of mitotic sister chromatid separation
positive regulation of attachment of mitotic spindle microtubules to kinetochore
positive regulation of mitotic cytokinesis
positive regulation of lateral attachment of mitotic spindle microtubules to kinetochore
mitotic sister chromatid biorientation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support