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Invitrogen
Auxin response factor Monoclonal Antibody (14D2)
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Auxin response factor Antibody (MA5-34937) in IHC (P)
Immunohistochemistry analysis of Auxin response factor in paraffin-embedded leafbud. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Auxin response factor monoclonal antibody (Product # MA5-34937) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (m... View More
产品信息
MA5-34937
产品规格
种属反应
Plant
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
14D2
抗原
Recombinant protein within tea Auxin response factor aa 401-586
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Control: Tea leafbud.
靶标信息
Auxin response factors (ARFs) are transcription factors that bind to auxin response elements in promoters of early auxin response genes and play central roles in many auxin-mediated processes. As transcription factors, ARFs possess a modular structure and consist of three major domains: an amino-terminal DNA-binding domain (DBD); a middle domain, which is responsible for gene activation or repression; and a carboxy-terminal PB1(Phox and Bem1) domain, contained within a region that was previously called domain III/I. ARFs function as transcription activators or repressors, depending on the middle region of the ARF protein; Q-rich middle regions activate transcription, whereas P/S/T-rich middle regions confer repressor activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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