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Invitrogen
B-Raf (G469A Mutant) Recombinant Rabbit Monoclonal Antibody (HL4008)
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B-Raf (G469A Mutant) Antibody (MA568377) in ICC/IF
Immunocytochemistry analysis of B-Raf (G469A Mutant) in Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Samples were incubated with B-Raf (G469A Mutant) recombinant monoclonal antibody (Product # MA568377) using a dilution of 1:500 (Green). Fluoroshield with DAPI (Blue).
产品信息
MA568377
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
HL4008
抗原
Carrier-protein conjugated synthetic peptide surrounding mutant G469T of human B-Raf. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
B-Raf is a proto-oncogene that encodes the B-Raf protein, a serine/threonine kinase involved in the MAPK/ERK signaling pathway, which regulates cell division, differentiation, and secretion. The G469A mutation in the B-Raf gene results in a substitution of glycine with alanine at position 469, which significantly impacts protein function. This mutation is found in various cancers, including melanoma, thyroid cancer, and colorectal cancer, and is known to enhance the kinase activity of B-Raf, leading to uncontrolled cell proliferation and survival. The G469A mutant displays increased catalytic activity compared to the wild-type B-Raf, contributing to oncogenic signaling. Research has focused on developing inhibitors that target this specific mutation to curb malignant growth. Structural studies indicate that the G469A mutation alters the conformation of the activation segment of B-Raf, rendering it constitutively active. Therapeutic approaches targeting B-Raf G469A mutation include kinase inhibitors that selectively inhibit the mutant's enhanced activity, offering potential for targeted cancer therapy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 94 kDa B-raf protein; B-Raf G469A; B-Raf proto-oncogene serine/threonine-protein kinase (p94); murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1
基因别名: B-raf; B-RAF1; BRAF; BRAF1; NS7; RAFB1
UniProt ID: (Human) P15056
Entrez Gene ID: (Human) 673
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
calcium ion binding
protein binding
ATP binding
small GTPase binding
mitogen-activated protein kinase kinase binding
identical protein binding
protein heterodimerization activity
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPKK activity
myeloid progenitor cell differentiation
protein phosphorylation
visual learning
organ morphogenesis
positive regulation of gene expression
negative regulation of fibroblast migration
glucose transport
thyroid gland development
positive regulation of peptidyl-serine phosphorylation
somatic stem cell population maintenance
cellular response to drug
regulation of cell proliferation
negative regulation of apoptotic process
CD4-positive, alpha-beta T cell differentiation
positive T cell selection
response to peptide hormone
negative regulation of neuron apoptotic process
thymus development
positive regulation of axon regeneration
positive regulation of axonogenesis
protein heterooligomerization
positive regulation of stress fiber assembly
response to cAMP
long-term synaptic potentiation
head morphogenesis
face development
positive regulation of ERK1 and ERK2 cascade
cellular response to calcium ion
establishment of protein localization to membrane
positive regulation of substrate adhesion-dependent cell spreading
negative regulation of synaptic vesicle exocytosis
negative regulation of endothelial cell apoptotic process
