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Identifyn
BAP1.p SRM Polyclonal Antibody, Alexa Fluor™ 488, Identifyn™
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BAP1.p SRM Antibody (DC-000049) in ICC/IF
Identifyn Detailed protocols can be found below under Documents & Downloads. Super Resolution - SIM2 Microscopy: HeLa cells were grown on acid-etched 1.5H cover glass to ~50% confluency. DNA damage was induced via treatment with 2 µM Etoposide-spiked complete growth media for 24 hours, followed by -20°C methanol fixation and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary anti... View More
产品信息
DC-000049
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide Sequence including the Phosphorylation of Serine 592 (S592), referencing both Uniprot ID Q92560 and Gene ID 8314.
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 10% glycerol
内含物
0.02% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:250-1:1,000
Widefield SIM - 1:250-1:1,000
Confocal - 1:250-1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) - 1:1,000
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000
Detailed protocols can be found below under Documents & Downloads.
靶标信息
BRCA1-associated protein-1, or BAP1, interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns. BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4. 8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21. 3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas. Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); BRCA1-associated protein 1; Cerebral protein 6; cerebral protein-13; DKFZp686N04275; FLJ35406; FLJ37180; Ubiquitin carboxyl-terminal hydrolase BAP1
基因别名: BAP1; HUCEP-13; hucep-6; KIAA0272; UCHL2
UniProt ID: (Human) Q92560
Entrez Gene ID: (Human) 8314
regulation of cell growth
cellular protein modification process
ubiquitin-dependent protein catabolic process
negative regulation of cell proliferation
protein deubiquitination
monoubiquitinated protein deubiquitination
monoubiquitinated histone H2A deubiquitination
regulation of cell cycle
protein K48-linked deubiquitination
positive regulation of protein targeting to mitochondrion
