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Agrisera
BIK1/Botrytis-induced kinase 1 Polyclonal Antibody
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BIK1/Botrytis-induced kinase 1 Antibody (AS16-4030) in WB
Total protein from 7-day old Arabidopsis thaliana seedlings Col-0 (wild-type) and bik 1 (null mutant) (Lu et al, 2009) were extracted with 50 m M HEPES-KOH buffer containing 250 m M sucrose, 5% glycerol, 50 m M Na PP, 1 m M Na Mo, 25 m M Na F, 10 m M EDTA, 0.5% PVP, 3 m M DTT, 1 m M PMSF, 10 u M Leupeptin & 10 n M Calyculin, and then fractionated by ultracentrifugation at 100,000 x gravity for 30 min at 4°C... View More
产品信息
AS16-4030
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BIK1 sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
BIK1, also called Botrytis induced kinase 1, is a receptor like cytoplasmic kinase in plants that functions downstream of pattern recognition receptors to transduce immune signals. It associates with receptor complexes such as FLS2 and EFR and is phosphorylated upon detection of microbial patterns, then phosphorylates NADPH oxidases and other targets to trigger reactive oxygen species production, calcium influx, MAP kinase activation, and transcriptional reprogramming. BIK1 also integrates hormone pathways, including salicylic acid, jasmonate, and ethylene, and contributes to resistance against diverse pathogens while influencing growth traits like root development and brassinosteroid signaling. Its activity is controlled by phosphorylation status, ubiquitination, and interaction with regulatory proteins such as PBS1 like kinases, and its stability is modulated by pathogen effectors. Mutations in BIK1 alter basal immunity, reactive oxygen burst, and susceptibility to pathogens, highlighting its central role in early immune signaling at the plant cell surface.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT2G39660; botrytis-induced kinase1; F12L6.32; F12L6_32
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