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Invitrogen
BMAL1 Polyclonal Antibody
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图: 1 / 5
BMAL1 Antibody (PA1-523) in ICC/IF
Immunofluorescent analysis of BMAL1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with BMAL1 Rabbit Polyclonal Antibody (Product # PA1-523) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA1-523
应用
建议稀释比
已发表文章
产品规格
种属反应
Hamster,
Human,
Mouse,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide: C D(582) M I D N D Q G S S S P S(594)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
200 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-523 detects BMAL1/aryl hydrocarbon nuclear translocator 3 (ARNT3) from hamster and mouse tissues as well as recombinant human BMAL1.
PA1-523 has been sucessfully used in Western blot procedures. By Western blot, this antibody detects a 110 kDa protein which corresponds to the product of a hamster GST-BMAL1 fusion construct overexpressed in E. coli. This antibody detects a non-specific band in U87-MG cell lysates at ~105kDa and in NIH-3T3 cell lysates at ~135kDa.
PA1-523 immunizing peptide corresponds to amino acid residues 582-594 from mouse BMAL1. This sequence is completely conserved between mouse, rat, guinea pig, and human BMAL1. PA1-523 immunizing peptide (Cat. # PEP-075) is available for use in neutralization and control experiments.
靶标信息
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ARNT-like protein 1, brain and muscle; ARNTL; aryl hydrocarbon receptor nuclear translocator like; aryl hydrocarbon receptor nuclear translocator-like; aryl hydrocarbon receptor nuclear translocator-like protein 1; basic helix-loop-helix family member e5; basic-helix-loop-helix-PAS orphan MOP3; basic-helix-loop-helix-PAS protein MOP3; bHLH-PAS protein JAP3; bHLH/PAS domains; bmal1; BMAL1h; brain and muscle ARNT-like 1; CG8727 PA; class E basic helix-loop-helix protein 5; cycle; member of PAS protein 3; member of PAS superfamily 3; MGC47515; mutant basic helix-loop-helix ARNT-like protein 1; PAS domain containing 3; PAS domain-containing protein 3; testis tissue sperm-binding protein Li 50e; tic; unnamed protein product
基因别名: Arnt3; ARNTL; ARNTL1; bHLHe5; BMAL1b; bmal1b'; BMAL1c; JAP3; MOP3; PASD3; TIC
UniProt ID: (Human) O00327, (Rat) Q9EPW1, (Mouse) Q9WTL8
Entrez Gene ID: (Chinese hamster) 100755141, (Human) 406, (Rat) 29657, (Mouse) 11865
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription cofactor activity
protein binding
aryl hydrocarbon receptor binding
sequence-specific DNA binding
protein dimerization activity
Hsp90 protein binding
E-box binding
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
bHLH transcription factor binding
protein heterodimerization activity
basic helix-loop-helix transcription factor
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
spermatogenesis
circadian rhythm
negative regulation of TOR signaling
circadian regulation of gene expression
regulation of hair cycle
positive regulation of circadian rhythm
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of fat cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of neurogenesis
regulation of insulin secretion
regulation of cell cycle
response to redox state
positive regulation of canonical Wnt signaling pathway
oxidative stress-induced premature senescence
energy homeostasis
negative regulation of cold-induced thermogenesis
positive regulation of protein acetylation
regulation of type B pancreatic cell development
negative regulation of glucocorticoid receptor signaling pathway
regulation of cellular senescence
positive regulation of skeletal muscle cell differentiation
protein import into nucleus
autophagy
gene expression
regulation of protein catabolic process
maternal process involved in parturition
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