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Invitrogen
BMI-1 Polyclonal Antibody
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图: 1 / 3
BMI-1 Antibody (PA1-32487) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of BMI-1 in methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h prior to incubation with the BMI-1 Polyclonal Antibody (Product # PA1-32487) (1:200 dilution) overnight at 4°C and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA1-32487
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to amino acids 252-264 of human Bmi1 protein.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.91 mg/mL
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Pcgf4 antibody; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51; RP11-573G6.1
基因别名: BMI1; PCGF4; RNF51
Entrez Gene ID: (Human) 648
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
