Invitrogen

BOP1 Polyclonal Antibody

查看其他7个BOP1抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

BOP1 Antibody in Immunocytochemistry (ICC/IF)

BOP1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

BOP1 Antibody (PA5-117811) in ICC/IF

Immunofluorescence staining of BOP1 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with BOP1 Polyclonal Antibody (Product # PA5-117811, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.

BOP1 Antibody in Immunocytochemistry (ICC/IF)

BOP1 Antibody in Western Blot (WB)

BOP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

BOP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of BOP1 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with BOP1 Polyclonal Antibody (Product # PA5-117811, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.

BOP1 Antibody in Western Blot (WB) — Western Blot using BOP1 Polyclonal Antibody (Product # PA5-117811) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 94 kDa. Observed band size: 100 kDa. (We are unsure of the identity of these extra bands).

BOP1 Antibody in Immunoprecipitation (IP) — BOP1 Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with BOP1 Polyclonal Antibody (Product # PA5-117811) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: BOP1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 94 kDa. Observed band size: 100 kDa.

产品信息

PA5-117811

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

免疫沉淀 (IP)

1-5 µL/mg of lysate

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human BOP1 fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7

内含物

0.03% ProClin 300

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Block of proliferation 1 protein; Ribosome biogenesis protein BOP1

查看更多收起

基因别名: BOP1; KIAA0124

查看更多收起

UniProt ID: (Human) Q14137

查看更多收起

Entrez Gene ID: (Human) 23246

查看更多收起

protein binding ribonucleoprotein complex binding poly(A) RNA binding ribosomal protein

cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA processing cell proliferation ribosome biogenesis regulation of cell cycle