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Invitrogen
BRD4 Recombinant Rabbit Monoclonal Antibody (BL-149-2H5)
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BRD4 Antibody (MA5-44203) in WB
Western blot analysis of BRD4 was performed by loading 30 µg of HeLa Wild Type (Lane 1) and HeLa BRD4 KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with BRD4 Recombinant Rabbit Monoclonal Antibody (7L7M8) (Product # MA5-44203, 1:1,000) and detected by Goat anti-Rabbit IgG (H+L) Superclonal... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-44203
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
BL-149-2H5
抗原
Synthetic peptide representing a region between residues 1312 and 1362 of human bromodomain-containing protein 4.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
100 µg/mL
规格
10 µg
纯化类型
purified
保存液
PBS, pH 8.2, with 0.1% BSA
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
靶标信息
BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BRD4-NUT FUSION; chromosome-associated protein; female sterile homeotic related gene 4; HUNKI; mitotic chromosome-associated protein
基因别名: CAP; CDLS6; FSHRG4; HUNK1; HUNKI; MCAP
UniProt ID: (Human) O60885
Entrez Gene ID: (Human) 23476
transcription regulatory region sequence-specific DNA binding
p53 binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
protein serine/threonine kinase activity
protein binding
RNA polymerase II carboxy-terminal domain kinase activity
enzyme binding
protein binding, bridging
histone binding
RNA polymerase II C-terminal domain binding
P-TEFb complex binding
histone H4 reader activity
histone H4K12ac reader activity
histone H4K5ac reader activity
histone H4K16ac reader activity
histone H4K8ac reader activity
histone H3K9ac reader activity
histone H3K27ac reader activity
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation by host of viral transcription
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
negative regulation of DNA damage checkpoint
positive regulation of T-helper 17 cell lineage commitment
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