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Bethyl Laboratories
BRD4 Recombinant Rabbit Monoclonal Antibody (BL-151-6F11)
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BRD4 Antibody (A700-005) in ICC/IF
Detection of human BRD4 by immunocytochemistry. Sample: formaldehyde-fixed Hep-G2 cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (Product # A700-005) (lot 1) used at 1:50. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG antibody (A120-201D4).
产品信息
A700-005
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
BL-151-6F11
抗原
Synthetic peptide representing a region between residues 1312 and 1362 of human bromodomain-containing protein 4.
偶联物
形式
Liquid
浓度
0.10 mg/mL
纯化类型
Ion-exchange chromatography
保存液
BBS, pH 8.2, with 0.1% BSA
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For ICC, formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
靶标信息
BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BRD4-NUT FUSION; bromodomain-containing 4; bromodomain-containing 5; Bromodomain-containing protein 4; chromosome-associated protein; fsh-like protein; MCAP; Mitotic chromosome-associated protein; Protein HUNK1
基因别名: BRD4; Brd5; CAP; HUNK1; HUNKI; MCAP; WI-11513
UniProt ID: (Human) O60885, (Mouse) Q9ESU6
Entrez Gene ID: (Human) 23476, (Mouse) 57261
regulation of transcription involved in G1/S transition of mitotic cell cycle
transcription, DNA-templated
cellular response to DNA damage stimulus
positive regulation of G2/M transition of mitotic cell cycle
viral process
covalent chromatin modification
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
regulation of phosphorylation of RNA polymerase II C-terminal domain
inner cell mass cell proliferation
regulation of transcription, DNA-templated
protein phosphorylation
chromosome segregation
chromatin modification
positive regulation of DNA binding
histone H4-K12 acetylation
histone H3-K14 acetylation
