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Agrisera
BRI1/Brassinosteroid insensitive 1 Polyclonal Antibody
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BRI1/Brassinosteroid insensitive 1 Antibody (AS12-1859) in WB
15 µg of total protein from leaf material of 5 week-old plants of Arabidopsis thaliana, were extracted with homogenization buffer (250 m M sucrose, 50 m M HEPES-KOH p H 7.5,5% glycerol, 0.5% Triton X-100,50 m M Na 4 P 2 O 7,1 m M Na 2 Mo O 4,25 m M Na F, 2 m M DTT, Sigma plant protease inhibitor cocktail). 3 parts of protein extract were mixed with 1 part of standard SDS loading buffer (200 m M TRIS p H=6.8... View More
产品信息
AS12-1859
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BRI1 protein.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
BRI1 encodes a plasma membrane, single-pass transmembrane leucine-rich repeat receptor-like serine/threonine kinase that functions as a principal cell-surface receptor for brassinosteroid hormones in plants. The protein comprises an N-terminal signal peptide and a large extracellular ectodomain built from a superhelix of ~25 leucine-rich repeats that includes a distinctive ~70-amino-acid "island" insertion; this island and adjacent leucine-rich repeats form the steroid-binding pocket for brassinolide. A short transmembrane helix anchors the receptor, followed by a cytosolic kinase domain whose activation depends on phosphorylation and receptor complex formation. Upon brassinosteroid binding, BRI1 promotes assembly with a SERK-family co-receptor (notably BAK1), enabling reciprocal trans-phosphorylation of the cytoplasmic kinase domains and initiation of downstream signaling that alters transcriptional programs controlling broad aspects of growth and development (including cell expansion and developmental patterning).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT4G39400; ATBRI1; BIN1; BR INSENSITIVE 1; BRASSINOSTEROID INSENSITIVE 1; CABBAGE 2; CBB2; DWARF 2; DWF2; F23K16.30; F23K16_30
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