Search
Agrisera
BZR1/Brassinazole resistant 1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 1
BZR1/Brassinazole resistant 1 Antibody (AS22-4852) in WB
The total protein content of 7-day-old rice seedlings was extracted using an extraction buffer containing 50 m M Tris p H 7.5,150 Mm Na Cl, 0.5% Triton X-100, and a complete protease inhibitor (Sigma) (0.2 g of sample plus 100 mL of extraction solution). Cellular debris was removed by centrifugation at 4&(8)451;, 12,000 rpm for 5 minutes, and the supernatant was mixed with the 4×LaemmLi buffer (250 m M Tris... View More
产品信息
AS22-4852
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Oryza sativa BZR1 protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Oryza sativa
靶标信息
BZR1 encodes a nucleus-localized, brassinosteroid-responsive transcription factor that functions as a central downstream effector of the plasma-membrane brassinosteroid receptor pathway, shuttling between cytoplasm and nucleus in a phosphorylation-dependent manner. The Arabidopsis BZR1 protein is ~336 aa and contains an N-terminal BES1_N/BZR1-type DNA-binding domain, multiple regulatory phosphorylation sites targeted by the GSK3-like kinase BIN2, and a PEST-rich region implicated in protein turnover and regulatory interactions. In low brassinosteroid conditions, BIN2 phosphorylation reduces BZR1 activity by diminishing DNA binding, promoting association with 14-3-3 proteins that favors cytoplasmic retention, and increasing proteasome-mediated degradation; upon pathway activation, BZR1 becomes dephosphorylated (notably by PP2A), accumulates in the nucleus, and directly modulates brassinosteroid-responsive gene expression programs that drive growth and developmental outputs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: AT1G75080; BIS2; BRASSINAZOLE-RESISTANT 1; F9E10.7; F9E10_7
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
