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Invitrogen
Baculovirus Envelope gp64 Monoclonal Antibody (AcV1), eBioscience™
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Baculovirus Envelope gp64 Antibody (14-6991-82) in Flow
Staining of baculovirus-infected insect cells with 0.25 µg of Mouse IgG2a kappa Isotype Control Purified (Product # 14-4724-82) (blue) or 0.25 µg of Anti-Baculovirus Envelope gp64 Protein Purified (purple) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Total viable cells were used for analysis.
产品信息
14-6991-82
产品规格
种属反应
Virus
已发表种属
Virus
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
AcV1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The AcV1 monoclonal antibody reacts with the gp64 envelope protein of the baculovirus Autographa californica (AcMNPV). The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection. The AcV1 antibody can be used in flow cytometry-based viral titration experiments.
Applications Reported: The AcV1 antibody has been reported for use in flow cytometric analysis. This antibody can be used in identifying virally-infected insect cells.
Applications Tested: The AcV1 antibody has been tested by flow cytometry on baculovirus infected insect cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Baculoviruses are known to infect invertebrates, with over 600 species having been described. Immature (larval) forms of moth species are the most common hosts, but these viruses have also been found infecting sawflies, mosquitoes, and shrimp. Although baculoviruses are capable of entering mammalian cells in culture, they are not known to be capable of replication in mammalian or other vertebrate animal cells. The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
