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          • Primary Antibodies ›
          • BCL-2 Antibodies

          Invitrogen

          Bcl-2 Recombinant Rabbit Monoclonal Antibody (B9E6)

          查看其他106个BCL-2抗体

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          Cite Bcl-2 Recombinant Rabbit Monoclonal Antibody (B9E6)

          Additional Information:
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          • 抗体检测数据 (11)
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          图: 1 / 11

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          Bcl-2 Antibody (MA572391) in IHC (P)

          Immunohistochemistry analysis of Bcl-2 on paraffin-embedded Human Lung Cancer tissue. Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15 min. Samples were incubated with Bcl-2 Recombinant Monoclonal (Product # MA572391) using a dilution at 1:200 overnight at 4°C. Followed by DAB staining. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Western Blot (WB)
          Bcl-2 Antibody in Western Blot (WB)

          产品信息

          MA572391

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500-1:2,000
          -

          免疫组化(石蜡) (IHC (P))

          1:100-1:500
          -

          免疫组化(冰冻) (IHC (F))

          1:100-1:500
          -
          产品规格

          种属反应

          Human, Mouse

          宿主/亚型

          Rabbit / IgG

          Expression System

          HEK293 cells

          分类

          Recombinant Monoclonal

          类型

          Antibody

          克隆号

          B9E6

          抗原

          KLH conjugated synthetic peptide derived from human Bcl-2.

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          规格

          100 µg

          纯化类型

          Protein A

          保存液

          TBS, pH 7.4, with 50% glycerol, 1% BSA

          内含物

          0.02% ProClin 300

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Ambient (domestic); Wet ice (international)

          靶标信息

          BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

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          引用该产品

          生物信息学

          蛋白别名: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; bcl-2 protein; bcl2-alpha protein; bcl2-beta protein; bcl2-Ig fusion gene; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

          查看更多 收起

          基因别名: AW986256; Bcl-2; BCL2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PPP1R50

          查看更多 收起

          UniProt ID: (Human) P10415, (Mouse) P10417

          查看更多 收起

          Entrez Gene ID: (Human) 596, (Mouse) 12043

          查看更多 收起

          功能
          protease binding protein binding channel activity channel inhibitor activity protein phosphatase binding ubiquitin protein ligase binding identical protein binding sequence-specific DNA binding protein heterodimerization activity BH3 domain binding protein phosphatase 2A binding binding, bridging DNA-binding transcription factor binding transcription factor binding protein homodimerization activity BH domain binding
          参与通路
          G1/S transition of mitotic cell cycle protein polyubiquitination mitotic cell cycle cell morphogenesis ossification ovarian follicle development metanephros development ureteric bud development branching involved in ureteric bud morphogenesis behavioral fear response leukocyte homeostasis B cell homeostasis B cell apoptotic process kidney development release of cytochrome c from mitochondria regulation of cell-matrix adhesion lymphocyte homeostasis lymphoid progenitor cell differentiation B cell lineage commitment T cell lineage commitment immune system development negative regulation of B cell apoptotic process response to ischemia renal system process melanin metabolic process regulation of nitrogen utilization cellular calcium ion homeostasis autophagy apoptotic process humoral immune response cellular response to DNA damage stimulus response to oxidative stress actin filament organization axonogenesis female pregnancy cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation male gonad development extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway in response to oxidative stress apoptotic mitochondrial changes response to radiation response to xenobiotic stimulus response to toxic substance post-embryonic development organ morphogenesis response to iron ion response to UV-B response to gamma radiation regulation of gene expression regulation of autophagy negative regulation of autophagy negative regulation of calcium ion transport into cytosol regulation of glycoprotein biosynthetic process mesenchymal cell development positive regulation of neuron maturation smooth muscle cell migration positive regulation of smooth muscle cell migration cell migration cochlear nucleus development gland morphogenesis hemopoiesis B cell differentiation T cell differentiation negative regulation of ossification positive regulation of cell growth negative regulation of cell growth melanocyte differentiation negative regulation of cell migration positive regulation of B cell proliferation hair follicle morphogenesis axon regeneration regulation of protein stability endoplasmic reticulum calcium ion homeostasis glomerulus development negative regulation of cellular pH reduction regulation of protein localization myeloid cell apoptotic process negative regulation of myeloid cell apoptotic process T cell differentiation in thymus osteoblast proliferation negative regulation of osteoblast proliferation response to cytokine response to nicotine organ growth regulation of growth positive regulation of multicellular organism growth B cell proliferation cellular response to glucose starvation response to hydrogen peroxide neuron maturation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process regulation of programmed cell death CD8-positive, alpha-beta T cell lineage commitment pigmentation negative regulation of neuron apoptotic process ear development regulation of viral genome replication positive regulation of melanocyte differentiation negative regulation of mitotic cell cycle retinal cell programmed cell death negative regulation of retinal cell programmed cell death regulation of mitochondrial membrane permeability focal adhesion assembly developmental pigmentation regulation of developmental pigmentation positive regulation of developmental pigmentation spleen development thymus development response to steroid hormone digestive tract morphogenesis developmental growth oocyte development skeletal muscle fiber development positive regulation of skeletal muscle fiber development pigment granule organization stem cell development homeostasis of number of cells within a tissue B cell receptor signaling pathway response to glucocorticoid neuron apoptotic process defense response to virus establishment of localization in cell regulation of cell cycle regulation of mitochondrial membrane potential negative regulation of mitochondrial depolarization regulation of calcium ion transport hematopoietic stem cell differentiation calcium ion transport into cytosol intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress lymphocyte apoptotic process negative regulation of lymphocyte apoptotic process T cell apoptotic process negative regulation of T cell apoptotic process cellular response to hypoxia reactive oxygen species metabolic process dendritic cell apoptotic process motor neuron apoptotic process extrinsic apoptotic signaling pathway in absence of ligand cell-cell adhesion negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of intracellular signal transduction epithelial cell apoptotic process negative regulation of epithelial cell apoptotic process negative regulation of G1/S transition of mitotic cell cycle negative regulation of dendritic cell apoptotic process negative regulation of motor neuron apoptotic process negative regulation of anoikis regulation of apoptotic signaling pathway negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway response to acid chemical protein dephosphorylation cell aging cell growth single organismal cell-cell adhesion peptidyl-serine phosphorylation peptidyl-threonine phosphorylation regulation of transmembrane transporter activity positive regulation of peptidyl-serine phosphorylation growth response to drug positive regulation of catalytic activity regulation of protein homodimerization activity regulation of protein heterodimerization activity regulation of catalytic activity transmembrane transport cellular response to organic substance negative regulation of reactive oxygen species metabolic process
          It has to be done as per old AB suggested Products section.

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