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Beclin 1 Polyclonal Antibody
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图: 1 / 4
Beclin 1 Antibody (600-401-MG4) in ICC/IF
Immunofluorescence of Rabbit Anti-Beclin 1 Antibody. Cells: HeLa cells. Fixative: 100% MeOH. Permeabilizaton: 0.3% Triton X-100. Primary Antibody: Anti-Beclin-1 at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5µg/mL for 60mins at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI, (B) Anti-Beclin1, (C) Merge A+B, (D) secondary only. Expected localization:: Golgi apparatus, mitochondria, endosome, nucleus.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
600-401-MG4
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-BECLIN1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human Beclin-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human Beclin-1. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, rat, bovine, pongo abelii, and wild boar and 92% to Gallus gallus.
靶标信息
Beclin 1 plays a central role in autophagy. It acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Different PI3K complex forms are believed to play a role in multiple membrane trafficking pathways - PI3KC3-C1 is involved in the initation of autophagosomes and PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis. Beclin 1 also plays a role in antiviral host defense.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: APG6; Bcl-2-interacting protein beclin; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; coiled-coil, myosin-like BCL2-interacting protein; Protein GT197
基因别名: 4921513J16Rik; 5430417M23Rik; Atg6; BECN1; GT197
UniProt ID: (Mouse) O88597
Entrez Gene ID: (Mouse) 56208
autophagosome assembly
cytokinesis
angiogenesis
autophagy
cellular response to nitrogen starvation
lysosome organization
cell cycle
mitotic metaphase plate congression
negative regulation of cell proliferation
positive regulation of autophagy
macroautophagy
positive regulation of macroautophagy
CVT pathway
regulation of cytokinesis
receptor catabolic process
cellular response to glucose starvation
negative regulation of apoptotic process
nucleophagy
late endosome to vacuole transport
neuron development
beta-amyloid metabolic process
regulation of catalytic activity
cell division
defense response to virus
response to other organism
negative regulation of cell death
mitophagy in response to mitochondrial depolarization
positive regulation of attachment of mitotic spindle microtubules to kinetochore
positive regulation of mitophagy
negative regulation of reactive oxygen species metabolic process
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