Search
Agrisera
Ble tag/Bleomycin resistance protein Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 1
Ble tag/Bleomycin resistance protein Antibody (AS22-4832) in WB
2.5 µg/well of total protein extracted freshly from Tetradesmus obliquus. Exact buffer components were: PBS and denatured with exact buffer components Nu PAGE™ LDS Sample Buffer (2 X) (Invitrogen) at 95°C 10 min. Samples were separated in Nu PAGE™ Bis-Tris Mini Protein Gels, 4–12%, (Thermo Scientific) and blotted for 3 mins to nitrocellulose (pore size of 0.2 um), using: i Blot 2. Blot was blocked with 7.5%... View More
产品信息
AS22-4832
产品规格
种属反应
Tag
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Bleomycin resistance protein from Streptoalloteichus hindustanus.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Ble tag
靶标信息
Bleomycin resistance protein is a small intracellular bleomycin-binding determinant found in several bacteria (including bleomycin-producing actinomycetes and mobile genetic elements), where it functions primarily by drug sequestration rather than enzymatic modification. Structural studies show a compact, predominantly alpha/beta protein of ~14 kDa (about 120-130 aa) that forms a stable homodimer via domain/arm exchange, creating a groove/crevice at the dimer interface that constitutes the principal bleomycin-binding site; in solution, the functional dimer can bind two bleomycin molecules, thereby preventing the antibiotic from engaging DNA and suppressing bleomycin-dependent DNA strand scission. In cellular contexts, bleomycin resistance mediated by this binding protein can be complemented by host DNA repair capacity, consistent with the idea that rapid intracellular neutralization of bleomycin reduces genotoxic burden and improves survival under exposure.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: ble; bleMBL; BLMA; BLMT; BRPMBL
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
