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Invitrogen
Bora Polyclonal Antibody
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Bora Antibody (PA5-89480) in WB
Western blot analysis of Bora in extracts of various cell lines. Samples were incubated with Bora Polyclonal antibody (Product # PA5-89480) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
产品信息
PA5-89480
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 260-559 of human BORA (NP_079084.4).
偶联物
形式
Liquid
浓度
0.97 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: KYSLGSITSP SPISSPTFSP IEFQIGETPL SEQRKFTVHS PDASSGTNSN GITNPCIRSP YIDGCSPIKN WSPMRLQMYS GGTQYRTSVI QIPFTLETQG EDEEDKENIP STDVSSPAMD AAGIHLRQFS NEASTHGTHL VVTAMSVTQN QSSASEKELA LLQDVEREKD NNTVDMVDPI EIADETTWIK EPVDNGSLPM TDFVSGIAFS IENSHMCMSP LAESSVIPCE SSNIQMDSGY NTQNCGSNIM DTVGAESYCK ESDAQTCEVE SKSQAFNMKQ DHTTQRCWMK TASPFQCSSP; Positive Samples: HT-29, HeLa
靶标信息
Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HsBora; Protein aurora borealis; RP11-342J4.2
基因别名: BORA; C13orf34
UniProt ID: (Human) Q6PGQ7
Entrez Gene ID: (Human) 79866
